The company will start resurfacing and patching works on the A76 in Sanquhar from Sunday, 23 October.
The scheme aims to improve the road surface for over 4,000 vehicles which use the route each day.
For safety reasons, a total closure will be in place between 7am – 7pm from Carronbridge to Cumnock bypass. Diversions will be in place.
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Andy Fraser, operating company representative, Scotland TranServ, said: "With more than 4,000 vehicles using this route each day it is essential that we carry out these resurfacing and patching works to ensure the integrity of the A76 at this location for the travelling public for years to come.
"To ensure minimal disruption work has been programmed at the weekend when traffic flows are lighter however I would urge motorists to leave plenty of time for their journeys."
(LM/CD)
